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Showing :
batch effect
•
reset
0
votes
0
replies
1.2k
views
which value from ruvadj in missMethyl can present methylation status
methylation
missmethyl
RUV
batch effect
7.4 years ago
RC
• 0
1
vote
5
replies
1.6k
views
Comparing RNA-Seq expression data from different sequencers (HiSeq3000 and NovaSeq6000) - are there methods for batch effect corrections?
deseq2
rna-seq
illumina
batch effect
updated 7.4 years ago by
Michael Love
43k • written 7.4 years ago by
JP Cartailler
▴ 40
1
vote
3
replies
884
views
Differentially expressed genes detection between three classes with a batch effect in DESeq2
deseq2
batch effect
differential gene expression
updated 7.4 years ago by
Michael Love
43k • written 7.4 years ago by
francesco.tabaro
• 0
2
votes
5
replies
2.3k
views
Compare groups of different RNAseq sets
batch effect
limma
updated 7.4 years ago by
Gordon Smyth
53k • written 7.4 years ago by
b.nota
▴ 370
3
votes
3
replies
1.8k
views
Difficulties with probable batch effect in paired blocked (treatment and placebo) experiment
deseq2
edger
limma
batch effect
paired design
7.5 years ago
Vladimir Krasikov
▴ 90
1
vote
4
replies
2.1k
views
Normalized counts after fitting linear model with batch effects - edgeR
edger
batch effect
normalized counts
updated 7.6 years ago by
Ryan C. Thompson
★ 7.9k • written 7.6 years ago by
Yahan
• 0
1
vote
3
replies
3.9k
views
Batch effect removal with Deseq2
deseq2
batch effect
removebatcheffect()
limma
updated 7.7 years ago by
Michael Love
43k • written 7.7 years ago by
lirongrossmann
▴ 80
0
votes
2
replies
1.3k
views
Single and double mutant DEG analysis using DESeq2
deseq2
batch effect
rnaseq
updated 7.8 years ago by
Michael Love
43k • written 7.8 years ago by
Cloud
• 0
1
vote
1
reply
2.0k
views
Batch effect removal before SC3?
SC3
batch effect
removebatcheffect
updated 7.8 years ago by
Vladimir Kiselev
▴ 150 • written 7.8 years ago by
muad.abdelhay
▴ 10
0
votes
3
replies
2.4k
views
How can I use DESeq2 for exploratory analysis after batch effect removal using RUVSeq?
deseq2
ruvseq
rna-seq
batch effect
updated 7.8 years ago by
Michael Love
43k • written 7.8 years ago by
dbouzo
• 0
1
vote
4
replies
3.6k
views
Remove batch effect using individuals as random in the model in Limma
batch effect
Coeficents non estimable
block
random effects
limma design matrix
updated 7.8 years ago by
Aaron Lun
★ 29k • written 7.8 years ago by
mheydarpour
▴ 10
0
votes
0
replies
1.4k
views
SVA/Combat without fitting an intercept
sva
combat
batch effect
limma
7.9 years ago
Eddie Imada
• 0
6
votes
3
replies
7.6k
views
deseq2 correcting for batch effects
deseq2
batch effect
removebatcheffect()
design matrix
updated 7.9 years ago by
Michael Love
43k • written 7.9 years ago by
Assa Yeroslaviz
★ 1.5k
0
votes
2
replies
1.5k
views
Discrepancy in extracting contrasts
deseq2
resultsnames
batch effect
contrast
updated 7.9 years ago by
Michael Love
43k • written 7.9 years ago by
pkachroo
▴ 10
0
votes
0
replies
1.2k
views
Combined strand specific and unstranded samples
strand
rnaseq
differential gene expression
batch effect
counts
8.0 years ago
lirongrossmann
▴ 80
0
votes
0
replies
1.6k
views
Batch correction with uncertain biological variables
sva
svaseq
batch effect
batch effects
batch effect correction
8.1 years ago
vedran.franke
• 0
1
vote
1
reply
1.8k
views
Scater normalizeExprs() batch effect
scater
batch effect
updated 8.1 years ago by
Aaron Lun
★ 29k • written 8.1 years ago by
Jenn
▴ 30
0
votes
8
replies
8.0k
views
Differences in PCA plot - rld versus vsd normalized data (DESeq2)
deseq2
PCA
batch effect
8.1 years ago
pkachroo
▴ 10
2
votes
1
reply
1.3k
views
Plotting Gene Expression Data From Multiple Batches
batch effect
microarray
plot
updated 8.2 years ago by
Jenny Drnevich
★ 2.0k • written 8.2 years ago by
tcalvo
▴ 100
2
votes
1
reply
2.8k
views
Correcting for Batch Effects Prior to Differential Gene Expression Analysis with limma
limma
batch effect
rna-seq
updated 8.3 years ago by
Aaron Lun
★ 29k • written 8.3 years ago by
adscheid3
• 0
2
votes
1
reply
1.4k
views
gene expression data analysis and batch effect methods
batch effect
gene expression
updated 8.3 years ago by
chris86
▴ 420 • written 8.3 years ago by
tati6406
• 0
0
votes
0
replies
1.2k
views
gene expression data analysis and batch effect methods
batch effect
gene expression
8.3 years ago
tati6406
• 0
0
votes
3
replies
2.0k
views
Using Combat function of SVA to remove known batch effect and using the result for down-stream analysis
sva
batch effect
deseq2
edgeR
updated 8.3 years ago by
Aaron Lun
★ 29k • written 8.3 years ago by
littlefishes20
▴ 10
0
votes
0
replies
1.4k
views
Possible confounding of variable of interest with batch covariate and Partial NA coefficients when including batch in limma linear model for microarr…
limma
batch effect
design matrix
microarray
confounding
8.4 years ago
svlachavas
▴ 840
0
votes
0
replies
1.6k
views
Putative batch effect assessment and correction for downstream DE analysis with microarray dataset
hta2.0
limma
batch effect
affymetrix microarrays
ComBat
8.4 years ago
svlachavas
▴ 840
3
votes
4
replies
3.8k
views
Single cells batch effects
RUV
single cell
batch effect
8.5 years ago
ribioinfo
▴ 100
3
votes
19
replies
4.7k
views
remove batch effect and adjusted model for 4 covariates in Limma
limma
batch effect
covariates
contrast
differential gene expression
8.5 years ago
mheydarpour
▴ 10
1
vote
1
reply
1.7k
views
Batch Correction and Normalization for champ.CNA
bioconductor
ChAMP
copy number
batch effect
normalization
updated 8.5 years ago by
rasmus.rydbirk
▴ 10 • written 8.5 years ago by
apmishra
• 0
0
votes
0
replies
1.4k
views
RPKM counts with SVA package & Combat with SVA?
batch effect
combat sva
sva
8.5 years ago
Ahdee
▴ 60
1
vote
2
replies
1.2k
views
Removinf Batch effect in EDGE-R
R
edger
batch effect
updated 8.6 years ago by
Aaron Lun
★ 29k • written 8.6 years ago by
kaihami
▴ 40
6
votes
4
replies
1.9k
views
limma batch question
limma
batch effect
8.6 years ago
jalali4
• 0
0
votes
0
replies
1.4k
views
Bach effect and PEER/RUV correction on large population
methylation
gene expression
sva
batch effect
ruv
8.7 years ago
carleshf
▴ 10
1
vote
6
replies
3.0k
views
limma + voom + sva + complex RNA-seq design?
limma
sva
batch effect
8.8 years ago
irenegallego
• 0
0
votes
2
replies
1.2k
views
can a batch effect be determined in edgeR with only 2 reps?
edger
rnaseq
batch effect
read length
8.8 years ago
Spollen, William G.
▴ 30
1
vote
2
replies
2.1k
views
is it possible to before and after batch effect removal from the data and see that batch effect removal actually worked
combat sva
batch effect
comba
updated 8.8 years ago by
Vegard Nygaard
▴ 120 • written 8.9 years ago by
amoltej
▴ 10
6
votes
4
replies
1.9k
views
Combining newer/older RNAseq data, batch correcting
rnaseq
limma
sva
batch effect
voom
updated 8.9 years ago by
Aaron Lun
★ 29k • written 8.9 years ago by
schrist1
▴ 10
1
vote
5
replies
1.3k
views
differential expression analysis in edgeR when you have 2 experiments and batch effect in 1 of the 2 experiments.
edger
batch effect
differential expression
8.9 years ago
sezimmer
• 0
0
votes
2
replies
1.9k
views
DESeq2 Model matrix design: Merging large RNAseq projects
deseq2
model.matrix
batch effect
rnaseq
8.9 years ago
arom2
• 0
0
votes
3
replies
2.1k
views
SVA package for RRBS - bisulfite methylation data
rrbs
batch effect
methylation
sva
updated 8.9 years ago by
dashbrook1
• 0 • written 10.1 years ago by
GFM
▴ 20
0
votes
3
replies
1.5k
views
Incorporate "batch" information for paired-design ?
RNA-Seq
deseq2
batch effect
9.0 years ago
g.atla
▴ 10
1
vote
1
reply
2.0k
views
Inspection of batch effect correction success with ComBat in R, regarding a merged dataset comprised of 4 different batches
affymetrix microarrays
sva
combat sva
batch effect
plotMDS
updated 9.0 years ago by
Jeff Leek
▴ 650 • written 9.1 years ago by
svlachavas
▴ 840
1
vote
2
replies
1.7k
views
Batch effect problem using SVA showing problems
SVA
combat sva
batch effect
updated 9.1 years ago by
Jeff Leek
▴ 650 • written 9.1 years ago by
amoltej
▴ 10
5
votes
8
replies
11k
views
Limma design with continuous and categorical covariates
limma
batch effect
covariates
microarray
updated 16 months ago by
hortense96
• 0 • written 9.1 years ago by
dmcleanuk
▴ 30
0
votes
0
replies
978
views
Diffbind: blocking factors in a contrast not including all samples (bug?)
diffbind
batch effect
9.1 years ago
Jon Manning
▴ 90
0
votes
3
replies
1.4k
views
using technical replicates to batch correct
batch effect
9.1 years ago
Juliet Hannah
▴ 360
14
votes
2
replies
3.8k
views
voom with combat
limma
voom
combat
batch effect
updated 9.1 years ago by
Gordon Smyth
53k • written 9.1 years ago by
eldronzhou
▴ 10
6
votes
9
replies
2.1k
views
Interpretation of DE analysis results of an Illumina microarray dataset, and possible batch effect size problem
limma
illumina
microarray
multifactorial design
batch effect
updated 9.2 years ago by
Gordon Smyth
53k • written 9.2 years ago by
Konstantinos Yeles
▴ 90
2
votes
3
replies
1.9k
views
RNAseq outlier is a critical sample
rnaseq
outlier
statistics
batch effect
updated 9.3 years ago by
Gordon Smyth
53k • written 9.3 years ago by
grastalt27
• 0
52
votes
20
replies
51k
views
Batch correction in DESeq2
rnaseq
batch effect
deseq2
combat
removebatcheffect()
updated 9.3 years ago by
adam
• 0 • written 9.8 years ago by
AB
▴ 110
0
votes
0
replies
1.4k
views
SVA: Using ComBat for synthetic genetic array data
combat sva
sga
combat
batch effect
9.4 years ago
John Townsend
▴ 20
125 results • Page
2 of 3
Recent ...
Replies
Comment: Heatmap of the Count Matrix
by
ArnulfoByrd
• 0
Perhaps PCA loadings offer a more comprehensive perspective. Now, thinking back, I recall struggling with a similar data reduction dilemma …
Answer: readBam vs read_bam_insert_metrics
by
James W. MacDonald
68k
Depends on what you mean by input. If you mean to ask if you use `readBam` to generate something to use as an argument to `read_bam_insert_…
Answer: How to construct a BSgenome object from a custom genome assembly using BSgenomeF
by
James W. MacDonald
68k
There is an example in the help page for `forgeBSgenomePkgFromTwobitFile`. Have you tried emulating that?
Answer: Guidance on limma for pseudobulk scRNA-seq with multi-factor design
by
Gordon Smyth
53k
What you describe sounds a good approach to me. I would also use sample weights (`sample.weights=TRUE` when running `voomLmFit`) and robust…
Comment: Can I set the normalizationFactors of DEseq2 object as 1 after using RUVg?
by
Michael Love
43k
I moved your post from an "answer" to a "comment". I don't know about `offst()` function, what is the scale or direction, or whether you a…
Votes
Answer: Limpa Pipeline Order
A: How barcode-plot enrichment is calculated?
A: How barcode-plot enrichment is calculated?
Answer: ROAST compare results with complex design
How to account for solvent controls in DESeq2 when comparing follicular vs luteal phase?
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